rabbit polyclonal antibody against s pneumoniae  (Novus Biologicals)

Journal: Communications Chemistry

Article Title: Creating unimolecular multivalent diversity in protein conjugates via the Passerini multicomponent bioconjugation with isocyanoproteins

doi: 10.1038/s42004-025-01827-1

Figure Lengend Snippet: Passerini bioconjugation for the assembly of multivalent glycoconjugates incorporating bacterial polysaccharide antigens . A Repeating unit of the pneumococcal serotype 14 (Pn14) and the meningococcal serogroup C (MenC) capsular polysaccharides (CPs). B SE-HPLC traces (TSK 5000 PW column) of the natural, fragmented and—for CPs Pn14—oxidized polysaccharides. C Schematic representation of the structures of the multivalent BSA-MenC-Pn14 glycoconjugates produced by the Passerini bioconjugation with isocyanoproteins. D Antigenicity evaluation by Dot blot assays of glycoconjugates 23, 24, 25 and 26 in comparison with isocyano-BSAs and modified CPs, using reference antibodies against BSA, S. pneumoniae and N. meningitidis . Native BSA and the natural CPs antigens were used as positive controls.

Article Snippet: Next, the membrane was incubated with the primary antibodies: anti-BSA rabbit polyclonal IgG antibody (1:1000, Invitrogen Ref A11133, Lot 2206803), anti- S. pneumoniae rabbit polyclonal IgG antibody (1:1000, Bio-Rad Ref 0300-0218, Lot 155340, reactive for serotype 14) and anti- N. meningitidis rabbit polyclonal IgG antibody (1:2000, Bio-Rad Ref 6600-5906, Lot 158003, reactive for serogroup C) in a 1% skim milk solution in TBS at 37 °C for 60 min. After washing the membrane three times for 5 min with the washing solution, it was incubated with an anti-rabbit IgG antibody conjugated to HRP (1:10 000, Santa Cruz SC2357) in TBS at 37 °C for 60 min.

Techniques: Produced, Dot Blot, Comparison, Modification

Journal: Frontiers in Pharmacology

Article Title: Respiratory virus-induced bacterial dysregulation in pediatric airway tissue and the dual actions of Echinacea in reducing complications

doi: 10.3389/fphar.2025.1579551

Figure Lengend Snippet: Efficacy of Echinaforce in reducing RSV-induced S. pneumoniae adhesion in pediatric EpiAirway tissue. (A) EpiAirway tissues cultured in an air-liquid interface (ALI) were stained with anti- S. pneumoniae antibody (green) and DAPI for nuclei, visualized at ×20 magnification. Representative images are shown for the following conditions: (A) Vehicle Control + S. pneumoniae , (B) RSV + S. pneumoniae , (C) RSV + EF 1:200 + S. pneumoniae , and (D) RSV + EF 1:400 + S. pneumoniae . (B) Bar chart shows S. pneumoniae adhesion under different conditions: uninfected tissue (infected with S. pneumoniae but not RSV), RSV-infected, and RSV-infected tissues treated with Echinaforce ® (EF) at 1:200 and 1:400 dilutions. Data represent ALI-cultured EpiAirway tissues, with statistical significance indicated (* p < 0.05; ** p < 0.01) ns = not significant.

Article Snippet: After permeabilization with 0.3% Triton X-100 and blocking with 3% BSA in PBST, sections were incubated overnight at 4°C with the primary antibodies: anti- S. pneumoniae (10 μg/mL, NB100-64570, Novus Biologicals) and anti-Hib (10 μg/mL, orb157421, Biorbyt).

Techniques: Cell Culture, Staining, Control, Infection

Journal: Frontiers in Pharmacology

Article Title: Respiratory virus-induced bacterial dysregulation in pediatric airway tissue and the dual actions of Echinacea in reducing complications

doi: 10.3389/fphar.2025.1579551

Figure Lengend Snippet: Blocking ICAM-1 and PAFr reduces S. pneumoniae adhesion to virus-infected NHBE cells. Anti-ICAM-1 and anti-PAFr antibodies significantly reduced S. pneumoniae adhesion in (A) RSV- and (B) HPIV3-infected NHBE cells, rather than with PV14 infection (C) . Results are expressed as mean values ±SD from three independent experiments Statistical significance: p < 0.01 (**), p < 0.0001 (****), “ns” indicates no significance.

Article Snippet: After permeabilization with 0.3% Triton X-100 and blocking with 3% BSA in PBST, sections were incubated overnight at 4°C with the primary antibodies: anti- S. pneumoniae (10 μg/mL, NB100-64570, Novus Biologicals) and anti-Hib (10 μg/mL, orb157421, Biorbyt).

Techniques: Blocking Assay, Virus, Infection

Journal: Frontiers in Pharmacology

Article Title: Respiratory virus-induced bacterial dysregulation in pediatric airway tissue and the dual actions of Echinacea in reducing complications

doi: 10.3389/fphar.2025.1579551

Figure Lengend Snippet: Efficacy of Echinaforce in reducing RSV-induced S. pneumoniae adhesion in pediatric EpiAirway tissue. (A) EpiAirway tissues cultured in an air-liquid interface (ALI) were stained with anti- S. pneumoniae antibody (green) and DAPI for nuclei, visualized at ×20 magnification. Representative images are shown for the following conditions: (A) Vehicle Control + S. pneumoniae , (B) RSV + S. pneumoniae , (C) RSV + EF 1:200 + S. pneumoniae , and (D) RSV + EF 1:400 + S. pneumoniae . (B) Bar chart shows S. pneumoniae adhesion under different conditions: uninfected tissue (infected with S. pneumoniae but not RSV), RSV-infected, and RSV-infected tissues treated with Echinaforce ® (EF) at 1:200 and 1:400 dilutions. Data represent ALI-cultured EpiAirway tissues, with statistical significance indicated (* p < 0.05; ** p < 0.01) ns = not significant.

Article Snippet: After 2 h at 37°C, unbound bacteria were removed by five DPBS washes, and the tissues were fixed with 10% formalin for 45 min. Adherent bacteria were quantified using specific primary antibodies: NB100-64570 for S. pneumoniae (Novus Biologicals, United States) and orb157421 for Hib (Biorbyt, United States).

Techniques: Cell Culture, Staining, Control, Infection

Journal: Frontiers in Pharmacology

Article Title: Respiratory virus-induced bacterial dysregulation in pediatric airway tissue and the dual actions of Echinacea in reducing complications

doi: 10.3389/fphar.2025.1579551

Figure Lengend Snippet: Blocking ICAM-1 and PAFr reduces S. pneumoniae adhesion to virus-infected NHBE cells. Anti-ICAM-1 and anti-PAFr antibodies significantly reduced S. pneumoniae adhesion in (A) RSV- and (B) HPIV3-infected NHBE cells, rather than with PV14 infection (C) . Results are expressed as mean values ±SD from three independent experiments Statistical significance: p < 0.01 (**), p < 0.0001 (****), “ns” indicates no significance.

Article Snippet: After 2 h at 37°C, unbound bacteria were removed by five DPBS washes, and the tissues were fixed with 10% formalin for 45 min. Adherent bacteria were quantified using specific primary antibodies: NB100-64570 for S. pneumoniae (Novus Biologicals, United States) and orb157421 for Hib (Biorbyt, United States).

Techniques: Blocking Assay, Virus, Infection

Journal: Scientific Reports

Article Title: Protective effect of interferon type I on barrier function of human airway epithelium during rhinovirus infections in vitro

doi: 10.1038/s41598-024-82516-2

Figure Lengend Snippet: Bacterial translocation during HRV 16- S. pneumoniae co-infection into the cell layer of pBECs in ALI culture pre-treated with IFN-β1a. ( a ) Representative orthogonal views of pBECs in ALI culture after 24 h IFN-β1a pre-treatment and 24 h + 8 h, 48 h + 8 h and 72 h + 8 h co-infection with HRV 16 and S. pneumoniae (SP), orthogonal views were generated using confocal images. Green: ZO-1, red: S. pneumoniae , blue: DAPI; A: apical side, B: basal side; scale bar: 20 µm. ( b ) Quantification of bacterial translocation into the cell layer, expressed as depth in % of average cell layer height. n = 4, data represent mean ± SD, * -IFN-β1a/ + HRV/ + S. pneumoniae vs. + IFN-β1a/ + HRV/ + S. pneumoniae , ** p ≤ 0.01. Two-Way ANOVA, Tukey’s Multiple Comparison Test.

Article Snippet: For co-staining of S. pneumoniae , cells were incubated with rabbit anti- S. pneumoniae (8 μg/mL, 1 h at room temperature, Bio-Rad Laboratories, Hercules) instead of the anti-p63 antibody.

Techniques: Translocation Assay, Infection, Generated, Comparison

Journal: Scientific Reports

Article Title: Parallel detection of multiple biomarkers in a point-of-care-competent device for the prediction of exacerbations in chronic inflammatory lung disease

doi: 10.1038/s41598-024-62784-8

Figure Lengend Snippet: Detection of model pathogen S. pneumoniae. ( A ) Slide arrays processed in open well format. ( B , C ) Measurement of increasing numbers of bacteria, captured by different amounts of antibody in individual spots. Analyte was applied either in PBST (B) or in saliva ( C ) (each N = 3). Graphs in the right panel depict results of linear regression analysis assessing the relationship between fluorescence read-out (relative fluorescence unists, RFU) and analyte concentration in the samples. Note that the highest concentration of bacteria (1 × 10 8 CFU/ml) has been omitted from all regression analyses.

Article Snippet: For capturing of S. pneumoniae on slide surfaces, anti-phosphorylcholine IgA κ, (clone TEPC 15; Merck, Darmstadt, Germany) and for detection, biotinylated rabbit anti- S. pneumoniae antibody (MBS324036, MyBioSource, San Diego, CA) were used.

Techniques: Bacteria, Fluorescence, Concentration Assay

Journal: Scientific Reports

Article Title: Parallel detection of multiple biomarkers in a point-of-care-competent device for the prediction of exacerbations in chronic inflammatory lung disease

doi: 10.1038/s41598-024-62784-8

Figure Lengend Snippet: Detection of markers on glass slides using a microfluidic device. ( A ) Slide with four reaction arrays carrying duplicate spots of capture antibodies for the detection of four different analytes. ( B ) Detection of analytes in individually spiked saliva samples (analyte concentration: 10 ng/ml) using a mixture of biotinylated detection antibodies. ( C ) Detection of analytes in mixed spiked saliva samples (IL-6 & IL-8: 10 ng/ml, TNF-α: 25 ng/ml, S. pneumoniae : 2 × 10 5 CFU/ml) using individual biotinylated detection antibodies in each reaction chamber.

Article Snippet: For capturing of S. pneumoniae on slide surfaces, anti-phosphorylcholine IgA κ, (clone TEPC 15; Merck, Darmstadt, Germany) and for detection, biotinylated rabbit anti- S. pneumoniae antibody (MBS324036, MyBioSource, San Diego, CA) were used.

Techniques: Concentration Assay